Expression of Testicular Estrogen Receptor Alpha in Rats Exposed to Subchronic Inhalation Exposure of Transfluthrin


  • Nanik Triana Kartikasari Internal Medicine Specialist Education I, Faculty of Medicine, Universitas Brawijaya, dr.Saiful Anwar General Hospital, Malang
  • Putu Moda Arsana Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, Faculty of Medicine, Universitas Brawijaya, Malang
  • Djoko Wahono Soeatmadji Endocrinology, Division of Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, Faculty of Medicine, Universitas Brawijaya, dr.Saiful Anwar General Hospital, Malang



transfluthrin, subchronic inhalation, endocrine-disrupting chemicals, expression of testicular estrogen receptor α


Endocrine Disrupting compounds (EDCs) are exogenous materials that can interfere with the functions of the endocrine system. Research has demonstrated that pyrethroid compounds affect endocrine function. Transfluthrin belongs to the pyrethroid group that is widely used. These compounds could interact with estrogen receptor α and may increase the expression of estrogen receptor α in rat testis. Aim: to determine the effect of subchronic inhaled transfluthrin exposure on the expression of estrogen receptor α (ERα). Method: we used 35 adult male Wistar rats as experimental animals, which were randomly divided into 5 groups (n=7 per group): negative control group (rats without treatment), solvent control group (rats exposed to n-hexane solvent), and three treatment groups of subchronic inhaled transfluthrin: 0.1 mg/ml (treatment 1),  0.2 mg/ml (treatment 2) and 0.4 mg/ml (treatment 3), treated for 60 days. The immunohistochemical (IHC) staining was used to assess the expression of ERα. Result: there was a tendency for an increase in ERα expression with the addition of a transfluthrin dose. ER α in negative control group, solvent control group, treatment 1 group, treatment 2 group, and treatment 3 group was 16.75 ± 5.01 %; 16.64 ± 13.00 %; 19.31 ± 6.52 %; 25.63 ± 7.08 %, and 28.79 ± 20.31 %; p>0.05, respectively. There was a weak positive correlation between the dose of transfluthrin with ERα expression (r = 0.38, p = 0.025). Conclusion: subchronic inhalation exposure of transfluthrin in rats showed a tendency to increased the expression of ERα (dose-dependent). There is a weak positive correlation between the dose of transfluthrin with ERα expression.                                                                                            


Tabb MM, Blumberg B. New Modes of Action for Endocrine-Disrupting Chemicals. Molecular Endocrinology. 2006; 20 (3): 475–482.[PMID:16037129 DOI:10.1210/me.2004-0513]

Diamanti-Kandarakis E, Bourguignon JP, Giudice LC, Hauser R, Prins GS, Soto AM, Zoeller RT, Gore AC. Endocrine-Disrupting-Chemicals: an Endocrine Society Scientific Statement. Endocrine Review. 2009; 30(4):293-342.[PMID:19502515DOI:10.1210/er.2009-0002]

Perry, Melissa J. Effects of Environmental and Occupational Pesticide Exposure on Human Sperm: A Systematic Review. Human Reproduction Update. 2008; 14(3):233-242. []

Lemaire G, Mnif W, Pascussi JM, Pillon A, Rabenoelina F, Fenet H, Gomez E, Casellas C, Nicolas JC, Cavaillès V, Duchesne MJ, Balaguer P. Identification of New Human Pregnane X Receptor Ligands among Pesticides Using a Stable Reporter Cell System. Toxicol Sci. 2006; 91:501-509.[PMID: 16565514 DOI:10.1093/toxsci/kfj173]

Vesin A, Glorennec P, Bot BL, Wortham H, Bonvallot N, Quivet E. Transfluthrin Indoor Air Concentration and Inhalation Exposure during Application of Electric Vaporizers. Environment International. 2013; 60:1-6.[PMID:23973617 DOI:10.1016/j.envint.2013.07.011]

Sharpe RM. Pathways of Endocrine Disruption during Male Sexual Differentiation and Masculinisation. Dalam Darbre PD Best Practice & Research Clinical Endocrinology & Metabolism: Endocrine Disrupters. 2006; 20 (1): 91-110. [PMID:16522522 DOI:10.1016/j.beem.2005.09.005]

Whitehead SA, Rice S. Endocrine-Disrupting Chemicals as Modulators of Sex Steroid Synthesis. Dalam Darbre P.D, Best Practice, and Research Clinical Endocrinology and Metabolism: Disrupters. 2006; 20 (1): 45-61.

Sakr SA. Pyrethroid Inhalation-Induced Hepatotoxicity in Albino Rats. Thesis. 2003. Umm Al-Qura University, Makkah, Saudi Arabia.

U.S. Environmental Protection Agency. Permethrin Facts (Reregistration Eligibility Decision Fact Sheet). 2006. Available atURL:

Netherlands Rapporteur Member State (RMS). Transfluthrin (insecticides, acaricides, and products to control other arthropods). 2014. p6-11.

Omotoso GO, Onanuga IO, Ibrahim RB. Histological Effects of Permethrin Insecticide on the Testis of Adult Wistar Rats. Ibnosina Journal of Medicine and Biomedical Science. 2014;6(3):125-129. [DOI: 10.4103/1947-489X.210373]

Erin K, Shanle, Xu Wei. Endocrine Disrupting Chemicals Targeting Estrogen Receptor Signalling: Identification and Mechanisms of Action. Chemical Research in Toxicology. 2011; 24(1): 6–19. [doi: 10.1021/tx100231n]

Cheek AO, McLachlan JA. Environmental Hormones and the Male Reproductive System. Journal of Andrology. 1998; 19 (1): 1-6.

Arieska D, Fauziyatul E, Masrukha L. Pengaruh Paparan Sub-kronik Senyawa Insektisida Transfluthrin Terhadap Differensiasi Seksual Tikus Putih Jantan (Rattus Norvegicus strain Wistar). 2009. Malang: Brawijaya.

U.S EPA. Toxicology Review of n-Hexane. In Support of Summary Information on the Integrated Risk Information System (IRIS). 2005: 22-57.

Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological Profile for n-Hexane. 1999: 37-40.

Du G, Shen O, Sun H, Fei J, Lu C, Song Let al. Assessing Hormone Receptor Activities of Pyrethroid Insecticides and Their Metabolites In Reporter Gene Assays. Toxicol Science. 2010; 116 (1): 58-66.

Lazar MA. Mechanism of Action of Hormones that Act on Nuclear Receptors. in: Williams Textbook of Endocrinology [Internet]. Philadelphia: Saunders. 2008; 11. [38-46].

Nkya TE, Akhouayri I, Kisinza W, David J-P. Impact of Environment on Mosquito Response to Pyrethroid Insecticides: facts, evidences and prospects. Insect Biochemistry and Molecular Biology. 2013;43:407-16. [PMID: 23123179 DOI:10.1016/j.ibmb.2012.10.006]

Soeatmadji DW. Abstract: Potential Effect of Transfuthrin as an Endocrine Disruptor Chemical on Estrogen, Androgen, and Thyroid Receptors: Insilico Study. Journal of the ASEAN Federation of Endocrine Societies. 2015; 30(2): 7.